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Image Search Results
Journal: bioRxiv
Article Title: Chiari II brain malformation is secondary to open spina bifida
doi: 10.1101/2025.01.06.631442
Figure Lengend Snippet: ( A ) Illustration of the Cre-LoxP cross used to generate mice with SB phenotype. Cdx2Cre drives Pax3 knockout in the body only (light green highlight). The head remains genetically wild-type. ( B, C ) Cdx2Cre driving mTmG reporter expression in the body of E10.5 (A, cyan) and E15.5 (B, green). Magenta in (B) indicates region of no Cdx2 expression. ( D, E ) Representative images of E15.5 control (non-Cre; Pax3 fl/fl ) and mutant ( Cdx2 cre ;Pax3 flfl ) embryos. White arrows indicate the location of the open SB lesion (in E). ( F ) Phenotypic developmental timeline of Cdx2 cre ;Pax3 flfl mice, with dotted line indicating extent of open AV lesion. All scale bars = 500 µm.
Article Snippet:
Techniques: Knock-Out, Expressing, Control, Mutagenesis
Journal: bioRxiv
Article Title: Chiari II brain malformation is secondary to open spina bifida
doi: 10.1101/2025.01.06.631442
Figure Lengend Snippet: Sagittal cryosections of control (n = 3) and SB (n = 3) embryos, immunostained for Pax3 (yellow) and nuclear-stained with DAPI (magenta). ( A, B ) Head sections: Pax3 expression is present in the neural tube (rhombencephalon) of both control and SB (yellow arrowheads). ( C, D ) Lower body: Pax3 expression is present in the spinal region of control (yellow arrowheads), but it is not detectable in the SB section, consistent with lower body Pax3 knockout. ( E ) The four genotypes resulting from the mating in , with % values from 10 litters, and number of embryos with normal or SB phenotypes. Note the exclusive occurrence and full penetrance of SB in Cdx2 cre ;Pax3 flfl embryos. Overall genotype ratios do not differ significantly from Mendelian expectation (p > 0.05). Scale bar = 500 µm for A-D.
Article Snippet:
Techniques: Control, Staining, Expressing, Knock-Out